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Laboratory Medicine

Test catalogue

Prostatic Specific Antigen, Total (PSA)

Test Name

Prostatic Specific Antigen, Total (PSA)

Gold Tube Specimen Type / Requirements

Container Type = Plasma (Na heparin)

Minimum Volume = 2mL Plasma

Tube must fill to at least ½ or greater of its vacuum capacity

Specimen Handling

Collection Instructions: Serum is the recommended sample. Serum collected with or without gel separator. Plasma samples should not be used. Ensure both samples and requisitions are lebeled properly.

Write your initials, date and time of collection on the collection label.

Laboratory Staff Instructions: Separate plasma as soon as possible. Turbid serum samples or samples containing particulate matter should be centrifuged prior to assay. Repeated freeze-thaw cycles have no effect on free PSA, total PSA, or % free PSA.

Transportation: Transport at room temperature ASAP. Place on ice if time of collection to time sample get to lab exceeds 2hrs

Stability: PSA is stable at room temperature for 24 hours, refrigerated for 5 days and frozen at -20°C for 5 months. Speciems to be held for longer than 5 months should be frozen at -70°C.

Sample Rejection Criteria: Plasma, unlabeled or Not Sufficient Quantity Samples will be rejected

Required Documentation

Turnaround Time (TAT)

1 day

Test Utilization

Reference Value

0.2 – 4.0 ug/L

Test Code



Two-site sandwich immunoenzymatic

Testing Location


Other Information

Last Updated: September 26, 2019