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Protein Electrophoresis (Serum M-Spike, M1, M2, M3 Protein)

Test Name

Protein Electrophoresis (Serum M-Spike, M1, M2, M3 Protein)

Gold Tube Specimen Type / Requirements

Container Type = 1 Gold Top (SST)

Minimum Volume = 2 ml serum

Tube must fill to at least ½ or greater of its vacuum capacity

Specimen Handling

Collection Instructions: Collect sample in gold top (SST) tube. Write your initials, date and time of collection on the collection label.

Laboratory Staff Instructions: Specimen must be labelled properly. Sperate as soon and possible and send refrigrated.

Rejection Criteria: Grossly hemolyzed, lipemic, Unlabeled or Not Sufficient Quantity Samples will be rejected. Avoid repeat freeze/Thaw cycles.

Stability: Stable 6hrs at room temperature refrigerated for 14 days 2-8°C.

Transportation: Send sample to core lab as soon as possible.

Required Documentation

Turnaround Time (TAT)

2 days

Reference Value

Albumin: 32-48 g/L
Alpha1 globulin: 2-5 g/L
Alpha2 globulin: 4-9 g/L
Beta globulin: 4-11 g/L
Gamma globulin: 6-16 g/L

Test Utilization

NOTE: There is limited utility in repeating serum protein electrophoresis (SPEP), urine protein electrophoresis (UPEP, or serum free light chains in short interval. If these tests have been completed in the last 20 days they will not be processed. Please contact the core lab x5082 if you have an exceptional circumstance that warrants repeat testing.

Test Code



Capillary Electrophoresis

Testing Location

Referred Out – ON, Canada (ROSJ03)

Other Information

Last Updated: June 26, 2020