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Laboratory Medicine

Test catalogue

5-Hydroxyindoleacetic acid, 24Hrs Urine (5-HIAA, 24Hrs Urine)

Test Name

5-Hydroxyindoleacetic acid, 24Hrs Urine (5-HIAA, 24Hrs Urine)

Plain 24 bottle Specimen Type / Requirements

Container Type = 24 hour urine bottle with 25 mL 6N Hydrochloric Acid

Minimum Volume = 10 mL urine from mixed urine Collection

Specimen Handling

Collection Instructions: 24-hour urine collected in a polyethylene container with 25 mL 6N Hydrochloric Acid. It is important that the urine is refrigerated during the collection. The pH of the urine must be < 4.0. Foods rich in serotonin (bananas, avocados, eggplant, pineapple, plums, tomatoes, hickory nuts and molusks) and medications that may affect metabolism of serotonin must be avoided at least 72h before and during collection of urine for 5HIAA.

Write your "start" and "end" date and time of collection on the collection label or requisition.

Lab: Instructions: Ensure sample containers is affixed with the corrosive and poison stickers before issuring to patients. Collection instructions which can be obtained from paradigm, must be handed to patients with the specimen container. State 24 hours volume and collection date and time in comments.

Stability: stable for 1 month refrigerated at 2-8°C and longer frozen.

Transportation: Send sample to SMH core lab as soon as possible.

Sample Rejection Criteria: Urine collected without acid, leaking specimen or pH > 5.0

Required Documentation

Turnaround Time (TAT)

7 days (Batched)

Test Utilization

Reference Value

< 50 umol/d

Test Code




Testing Location


Other Information

Last Updated: February 11, 2020