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Test performed at:

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Gentamicin, Pre

Test Name

Gentamicin, Pre

Red Tube with thin top Specimen Type / Requirements

Container Type = 1 Red Top

Minimum Volume = 3 ml Blood

Tube must fill to at least ½ or greater of its vacuum capacity

Specimen Handling

Collection Instructions: Serum collected in red top tube. Avoid gold top (SST).Serum collected in red top vacutainer without gel separator. Collect immediately before next dose. Wait until the 3rd dose before collecting samples for therapeutic monitoring. Write your initials, date and time of collection on the collection label or requisition.

Special Instructions Serum free from hemolysis collected without gel separator. Separate samples from cells as soon as possible. Repeated freezing and thawing of samples should be avoided

Rejection Criteria: Gel separator, Unlabeled or Not Sufficient Quantity Samples will be rejected.

Stability: stable at room temperature for 24h, refrigerated 1 week, frozen 2 weeks (avoid repeat freeze/thaw cycles)

Transportation: Send sample to core lab as soon as possible.

Required Documentation

Turn Around Time

1 hour STAT/Trauma,
4 hours routine

Reference Value

For traditional (“conventional”) dosing method.

Pre-dose: < 2.0 mg/L for adults and pediatrics.

When used for synergy in the treatment of gram positive endocarditis in adults.

Pre-dose: < 1.0 mg/L for adults

For extended interval (“once daily”) dosing method.

Pre-dose: < 1.0 mg/L for adults

Critical pre-dose: >2.0mg/L

Test code



Enzyme Immunoassay

Testing Location


Other Information

Last Updated: December 11, 2019