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Laboratory Medicine

Test catalogue

Direct Antiglobulin Test (DAT)

Test Name

Direct Antiglobulin Test (DAT, Coombs Test, Direct Coombs)

Pink Tube Specimen Type / Requirements

Container Type = 1 Pink Top

Minimum Venous Volume = 1 x 7 ml EDTA specimen (Pink Top)

Tube must fill to at least ½ or greater of its vacuum capacity

Specimen Handling

Collection Instructions: Collect sample in 1 Pink Top tube. Sample should be well mixed at time of collection. Requisition must have ordering physician, phlebotomist signature, collection date and time. Specimen must be clearly labeled with patient information. EDC required for prenatal patients. Please include diagnosis, transfusion and pregnancy history if applicable.

Stability: Stable at room temperature for 8 hrs.

Transportation: Send to Transfusion Medicine lab at room temperature as soon as possible.

Sample Rejection Criteria: Grossly hemolytic, icteric or lipemic specimens. Unlabeled and/or mislabeled specimens. Incomplete TM requisitions.

Required Documentation

Ensure requisition is complete with ordering physician name and phlebotomist signature, date and time of draw.

Turnaround Time (TAT)

TAT: STAT: 1 hour, Routine: 4-6 hours.

TAT may be longer if result is positive

Test Utilization

As Required

Reference Value


Test Code




Testing Location

SMH-Transfusion Medicine (Blood Bank)

Other Information

Last Updated: October 08, 2019