View information on our visitor policy. >>

Information about coming with a patient for their appointment, test or surgery. >>

Text size: a a a

Laboratory Medicine

Test catalogue

CSF Analysis (Glucose & Total Protein Volume)

Test Name

CSF Analysis (Glucose & Total Protein Volume)

CSF Tube Urine sample Specimen Type / Requirements

Container Type = Sterile, screw-capped transport tube or CSF tube.

Preferred volume to collect: 1.0 mL CSF

Minimum volume to collect: 0.5 mL CSF

Neonate volume to collect: 0.5 mL CSF

Tube 1: Cell count (Can share with cytology)
Tube 2: Glucose and protein, Oligoclonal Banding
Tube 3: Gram stain and culture (C+S) or Virology
Tube 4: Cell count or CSF Flow Cytometry.

Specimen Handling

Collection Instructions: Collect appropriate volumes of CSF in sequential order into four sterile, screw capped tubes consecutively numbered 1 to 4; Label container and requisition with patient information and specimen source. Maintan sterility. Specify which tube number to run test on.

Write your initials, date and time of collection on the collection label.

Laboratory Staff Instructions: Enter date and time collection in the LIS. Specify sample number in order comment.

Stability: Stable for 1 day at 15-25°C, at least 7 days at 2-8°C and for 6 months at -20°C or lower temperature

 Send to lab immediately for specimen processing.
 Do not send via the pneumatic tube system. Do not refrigerate.
 Do not leave specimen on the reception counter; give directly to laboratory staff.
 CSF specimens with CJD and other infectious requests: follow Transportation of Dangerous Goods (TDG) and  Unity Health Toronto protocols.

Required Documentation

Turnaround Time (TAT)

1 Day

Test Utilization

Reference Value

CSF Protein: 0.15 - 0.45g/L

CSF Glucose: 60% of serum glucose

Test Code



Isoelectric Focusing (IEF)

Testing Location


Other Information

Last Updated: February 24, 2020