Laboratory Medicine

Test catalogue

CD4 / CD8
Test Name CD4 / CD8
Specimen Type / Requirements Container Type = 1 Lavender top tube Minimum Volume = 1 mL
Specimen Handling Collection Instructions: Collect sample in a lavender top and send to core lab. Ensure sample is mixed adequately.Test done Monday to friday at 13:00 only. Sample needs to be in lab by 1pm on fridays. Do not draw on Fridays after 13:00 and on weekends. Write your initials, date and time of collection on the collection label. Stability: Stable for 24hrs at room temperature and 3 days refrigerated. Transportation: Transport at room temperature within 1 hour of collection. Lab Instrustions Ensure Samples is delivered together with requisition to the blue bin at flow Cytometry as soon as possible. Sample Rejection Criteria:Clotted sample, Grossly hemolyzed samples, Unlabeled or Not Sufficient Quantity Samples will be rejected. Samples collected after 1pm on friday until 30hrs before the start of the next business day will be rejected.
Required Documentation
Turnaround Time (TAT) 7 days	Test Utilization Once per week
Reference value CD4 absolute 535-1125 Cells/µL CD4 percentage 32-48 % CD8 absolute 300-810 Cells/µL CD8 percentage 18-35 % CD4/CD8 ratio 0.80-2.80 NA
Test Code CD4P	Methodology Flow Cytometry Immunophenotyping
Testing Location SMH-Haematology	Other Information Last Updated: December 01, 2020

CD4 / CD8

Test Name

CD4 / CD8

Specimen Type / Requirements

Container Type = 1 Lavender top tube

Minimum Volume = 1 mL

Specimen Handling

Collection Instructions: Collect sample in a lavender top and send to core lab. Ensure sample is mixed adequately.Test done Monday to friday at 13:00 only. Sample needs to be in lab by 1pm on fridays. Do not draw on Fridays after 13:00 and on weekends. Write your initials, date and time of collection on the collection label.

Stability: Stable for 24hrs at room temperature and 3 days refrigerated.

Transportation: Transport at room temperature within 1 hour of collection.

Lab Instrustions Ensure Samples is delivered together with requisition to the blue bin at flow Cytometry as soon as possible.

Sample Rejection Criteria:Clotted sample, Grossly hemolyzed samples, Unlabeled or Not Sufficient Quantity Samples will be rejected. Samples collected after 1pm on friday until 30hrs before the start of the next business day will be rejected.

Required Documentation

Turnaround Time (TAT)

7 days

Test Utilization

Once per week

Reference value

CD4 absolute 535-1125 Cells/µL

CD4 percentage 32-48 %

CD8 absolute 300-810 Cells/µL

CD8 percentage 18-35 %

CD4/CD8 ratio 0.80-2.80 NA

Test Code

CD4P

Methodology

Flow Cytometry Immunophenotyping

Testing Location

SMH-Haematology

Other Information

Last Updated: December 01, 2020