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Keenan Research Centre - Facilities & Resources

Bioimaging Centre - Equipment

Leica TCS SL Confocal Microscope | Bio-Rad Confocal Microscope | Nikon TE300 Microscope | Nikon E800 Microscope | Leica AS LMD Laser microdisection system

 

Bio-Rad Radiance Plus Confocal Microscope

Bio-Rad Radiance Plus Confocal MicroscopeThe BioRad Radiance is an upright confocal system attached to a Nikon E800 fluorescent microscope. It has three lasers, and uses a single excitation fire wire fiber. There is also a transmitted light imaging capability with a photo-diode detector.

Each laser has a separate confocal aperture. High spectral separation of simultaneous multi-channel imaging is achieved by the use of an AOTF – acousto-optical tunable filter.

The system uses Laser Sharp image acquisition and analysis program to captures images, display multichannel merged images, and produces 3D visualization of Z-series. Data is exported standard 2D and 3D image display formats.

Bio-Rad Radiance Plus Confocal Microscope ImageObjectives

Plan Fluor 4x / 0.13 NA / 17.1mm
Plan Apo 10x / 0.45 NA / 4.0mm
Plan Apo 20x / 0.75 NA / 1.0mm
Plan Fluor 40x / 1.30 NA / 0.20mm
Plan Apo 60x / 1.40 NA . 0.21mm
Fluor 20x W / 0.5 NA / 2mm
Fluor 40xW / 0.8 NA / 2mm

Lasers

Argon laser (488nm)
HeNe laser (543nm)
Red diode laser (638nm)