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Test performed at:

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Tobramycin, Post

Test Name

Tobramycin, Post

Red Tube with thin top Specimen Type/Requirements

Container Type = 1 Red Top (Adult) or 1 Red Microtainer (Pediatric)

Minimum Volume = 3mL Blood

Specimen Handling

Collection Instructions: Collect 3mL of blood in red top tube for adults and 1mL in red microtainer for babies. Avoid gold top (SST).

For traditional dosing, collect sample 30 minutes after end of dose infusion. Wait until the 3rd dose before collecting samples for therapeutic monitoring.

For extended dosing, after the 3rd dose collect a sample 8-12 hours after end of dose infusion. Record the exact time of sample and use nomogram for dosage adjustment if necessary.

For further assistance with interpretation contact unit pharmacist.

Record date and time of last dose. Write your initials, date and time of sample collection on the collection label or requisition.

If no time of collection is indicated with the specimen, the test will be ordered as a random level.

Laboratory Staff Instructions: Separate samples from cells as soon as possible. Repeated freezing and thawing of samples should be avoided

Rejection Criteria: Gel separator, plasma, grossly hemolyzed and icteric samples, Unlabeled or Not Sufficient Quantity samples will be rejected.

Stability: Stable for 4 h at room temperature, 1 week at 2-8°C and 1 month frozen.

Transportation: Send to Core Lab as soon as possible

Required Documentation

Turnaround Time (TAT)

1 hour for Trauma/Stat - 2 hours for routine

Reference Value

For traditional (“conventional”) dosing method.

½ hour post-dose: 4.0 – 10.0 mg/L (pediatrics 5.0-10.0 mg/L)

Toxic: >21.0 mg/L

Test Code

TOBP

Methodology

Competitive Binding Immunoenzymatic Assay

Testing Location

SMH-Biochemistry

Other Information

Last Updated: December 11, 2019