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Laboratory Medicine

Test catalogue

Complement - Total (CH50), Serum

Test Name

Complement - Total (CH50), Serum

Red Tube Specimen Type / Requirements

Container Type = 1 Red Top

Minimum Volume = 2mL serum

Tubes must fill to at least ½ or greater of its vacuum capacity

Specimen Handling

Collection Instructions: Collect sample in red without gel separator. Allow filled tube to clot at 22°C-37°C for 30-60 minutes. Write your initials, date and time of collection on the collection label.

Laboratory Staff Instructions: CH50 may be decreased by: delayed separation of serum, insufficient clotting, prolonged storage at room temperature, use of plasma, presence of immune complexes, cryoglobulins, bacteria or particulates. Centrifuge at room Temperature. Aliquot into storage tube and freeze as soon as possible. Store and send frozen.

Rejection Criteria: Unlabeled or samples of none sufficient quantity. Plasma, lipemic and grossly hemolyzed specimens are not suitable for analysis. Avoid freeze/thaw cycles.

Stability: Stable at room temperature for 8 hours, refrigerated for 48 Hrs and frozen at -20°C or colder for 20 days. Thaw samples only once.

Transportation: Send sample to core lab as soon as possible.

Required Documentation

Turnaround Time (TAT)

5 days

Test Utilization

Reference Value

42 - 95 Units/mL

Test Code

CH50

Methodology

Spectrophotometry

Testing Location

Referred Out – ON, Canada (ROICL02)

Other Information

Last Updated: March 03, 2020